Inhibition of Raf/MAPK signaling in Xenopus oocyte extracts by Raf-1-specific peptides.

Abstract

Raf-1 is an upstream element of the mitogen-activated protein kinase (MAPK) pathway which leads to cell proliferation and differentiation. In this study Raf-1 derived peptides comprising the conserved amino acid residues Arg89 and Ser259, involved in binding of activated Ras and 14-3-3 proteins, respectively, were shown to interfere with MAPK activation in extracts from immature Xenopus oocytes. Lipids prepared from oocyte extracts can stimulate MAPK in a Ras- and protein kinase C-independent manner. This lipid-induced MAPK activation is blocked by a Raf-1 derived peptide comprising Ser259.

Cite this paper

@article{Radziwill1996InhibitionOR, title={Inhibition of Raf/MAPK signaling in Xenopus oocyte extracts by Raf-1-specific peptides.}, author={Gerald Radziwill and U Steinhusen and Alex Aitken and Karin Moelling}, journal={Biochemical and biophysical research communications}, year={1996}, volume={227 1}, pages={20-6} }