Inhibition of RNAse A family enzymes prevents degradation and loss of silencing activity of siRNAs in serum.

@article{Haupenthal2006InhibitionOR,
  title={Inhibition of RNAse A family enzymes prevents degradation and loss of silencing activity of siRNAs in serum.},
  author={J{\"o}rg Haupenthal and Christina Baehr and Simone Kiermayer and Stefan Zeuzem and Albrecht Piiper},
  journal={Biochemical pharmacology},
  year={2006},
  volume={71 5},
  pages={702-10}
}
Small interfering RNAs (siRNA), RNA duplexes of approximately 21 nucleotides, offer a promising approach to specifically degrade RNAs in target cells by a process termed RNA interference. Insufficient in vivo-stability is a major problem of a systemic application of siRNAs in humans. The present study demonstrated that RNAse A-like RNAses degraded siRNAs in serum. The susceptibility of siRNAs towards degradation in serum was strongly enhanced by local clustering of A/Us within the siRNA… CONTINUE READING

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