Inhibition of Nuclear Protein Import by Nonhydrolyzable Analogues of GTP and Identification of the Small GTPase Ran / TC 4 as an Essential Transport Factor

@inproceedings{Melchior2002InhibitionON,
  title={Inhibition of Nuclear Protein Import by Nonhydrolyzable Analogues of GTP and Identification of the Small GTPase Ran / TC 4 as an Essential Transport Factor},
  author={Frauke Melchior and Bryce M. Paschal and Janice Evans and Lar ry Gerace},
  year={2002}
}
We have investigated a possible involvement of GTPases in nuclear protein import using an in vitro transport system involving digitonin-permeabilized cells supplemented with exogenous cytosol. Transport in this system was measured with a novel ELISAbased assay that allows rapid quantitative analysis. GTP~,S and other nonhydrolyzable analogues of GTP were found to rapidly inhibit the rate of in vitro nuclear import. Transport inhibition by GTP3,S was dependent on the concentrations of… CONTINUE READING
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