Inhibition of N-linked glycosylation of the human type 1α metabotropic glutamate receptor by tunicamycin: effects on cell-surface receptor expression and function

@article{Mody1999InhibitionON,
  title={Inhibition of N-linked glycosylation of the human type 1$\alpha$ metabotropic glutamate receptor by tunicamycin: effects on cell-surface receptor expression and function},
  author={Nayha Mody and Emmanuel Hermans and Stefan R. Nahorski and R. A. John Challiss},
  journal={Neuropharmacology},
  year={1999},
  volume={38},
  pages={1485-1492}
}
N-linked glycosylation of the human bradykinin B2 receptor is required for optimal cell-surface expression and coupling
TLDR
Results indicate that, for the human bradykinin B2 receptor, glycosylation is not required for optimal ligand binding, but plays an important role in cell-surface addressing and receptor function.
Three putative N‐glycosylation sites within the murine 5‐HT3A receptor sequence affect plasma membrane targeting, ligand binding, and calcium influx in heterologous mammalian cells
TLDR
It is concluded that N‐glycosylation is a critical step in the maturation, trafficking, and function of the murine 5‐HT3AR.
N‐linked glycosylation of the mGlu7 receptor regulates the forward trafficking and transsynaptic interaction with Elfn1
  • Daehun Park, Sunha Park, Y. H. Suh
  • Biology
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2020
TLDR
It is found that mGlu7 is N‐glycosylated at four asparagine residues in heterologous cells and rat cultured neurons, and it is demonstrated that N‐ Glycosylation is essential for forward transport and surface expression of mGLU7.
Regulation of GIP and GLP1 Receptor Cell Surface Expression by N-Glycosylation and Receptor Heteromerization
TLDR
It is shown that each of these Asn residues is glycosylated when either human receptor is expressed in Chinese hamster ovary cells and that functional expression of a GIP receptor mutant lacking N-glycosylation is rescued by co-expressed wild type GLP1 receptor, which, together with data obtained using Bioluminescence Resonance Energy Transfer, suggests formation of aGIP-GLP1 receptors heteromer.
Direct coupling of detergent purified human mGlu5 receptor to the heterotrimeric G proteins Gq and Gs
TLDR
It was found that purified mGlu5 was capable of activating the G protein Gs either upon stimulation with VU0424465 or glutamate, although the later induced a much weaker response.
Complex involvement of pertussis toxin-sensitive G proteins in the regulation of type 1alpha metabotropic glutamate receptor signaling in baby hamster kidney cells.
TLDR
Evidence is provided for a multifunctional coupling of mGlu1alpha to different types of G proteins, including PTX-sensitive G(i)-type G proteins that are involved in the negative control of phospholipase C activity while also influencing the rate of desensitization of the mGLU1alpha receptor.
Metabotropic Glutamate 1 Receptor: Current Concepts and Perspectives
TLDR
Recent advancements in the pharmacology and concepts about the intracellular transduction and pathophysiological role of mGlu1 receptors are discussed and earlier data is reviewed in view of these novel findings.
Structural, signalling and regulatory properties of the group I metabotropic glutamate receptors: prototypic family C G-protein-coupled receptors.
TLDR
This review will focus on the group I mGlu receptors, a subgroup of receptors that is widely and differentially expressed in neuronal and glial cells within the brain, and receptor activation has been implicated in the control of an array of key signalling events.
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Identification of the Sites of N-Linked Glycosylation on the Human Calcium Receptor and Assessment of Their Role in Cell Surface Expression and Signal Transduction*
TLDR
Immunoblot analyses and glycosidase digestion studies of the wild typeversus mutant receptors demonstrate that among 11 potential N-linked glycosylation sites on the hCaR, eight sites are actually utilized; gly cosylation of at least three sites is critical for cell surface expression of the receptor, but glycosolation does not appear to be critical for signal transduction.
Characterization of the Dimerization of Metabotropic Glutamate Receptors Using an N‐Terminal Truncation of mGluR1α
TLDR
The results show that although the N‐terminal domain of mGluR1α is sufficient for dimer formation, other domains in the molecule must regulate the process.
Effects of Human Type 1α Metabotropic Glutamate Receptor Expression Level on Phosphoinositide and Ca2+ Signalling in an Inducible Cell Expression System
TLDR
The results clearly indicate the advantages of working with an inducible expression system when studying the biochemical and pharmacological properties of the human mGlu1α receptor in transfected cells.
Effects of varying the expression level of recombinant human mGlu1α receptors on the pharmacological properties of agonists and antagonists
TLDR
Both the potency and efficacy of mGlu1α receptor agonists are influenced by expression level, whilst mGLU1 α receptor antagonist activities are independent of expression level.
The Metabotropic Glutamate Receptor mGluR4, but Not mGluR1α, Is Palmitoylated when Expressed in BHK Cells
TLDR
The palmitoylated, endogenously expressed G protein α‐subunit αq could be immunoprecipitated from [3H]palmitate‐labelled BHK cells expressing mGluR1α using a specific antipeptide antibody, but in the same cell lysates no detectable palmitate was found, suggesting that this mGLUR subtype, associated with stimulation of phospholipase C, is not palMIToylated.
Enhanced type 1alpha metabotropic glutamate receptor-stimulated phosphoinositide signaling after pertussis toxin treatment.
TLDR
Changing changes in the concentration-effect curves for mGluR agonists are consistent with a model in which the receptor associates with PTX-sensitive inhibitory (Gi/o) and PTx-insensitive stimulatory (Gq/11) G proteins that can each influence PIC activity.
Dimerization of the Extracellular Calcium-sensing Receptor (CaR) on the Cell Surface of CaR-transfected HEK293 Cells*
TLDR
This study employed epitope tagging, cell-surface biotinylation, and immunoprecipitation techniques to demonstrate that the functional CaR is expressed mostly in the form of a dimer on the surface of transfected human embryonic kidney (HEK293) cells.
Cloning and expression of a human metabotropic glutamate receptor 1 alpha: enhanced coupling on co-transfection with a glutamate transporter.
TLDR
It is suggested that co-expression of mGluR1 alpha with a glutamate transporter prevents desensitization of the receptor, thus achieving optimal coupling of the receptors with its effector system.
Novel inhibitory action of tunicamycin homologues suggests a role for dynamic protein fatty acylation in growth cone-mediated neurite extension
TLDR
It is shown that ongoing fatty acylation can be inhibited selectively by long-chain homologues of the antibiotic tunicamycin, a known inhibitor of N-linked glycosylation, suggesting an important role for dynamic protein acylations in growth cone- mediated extension of neuronal processes.
Heterodimerization is required for the formation of a functional GABAB receptor
TLDR
The cloning of a complementary DNA encoding a new subtype of the GABAB receptor (GABABR2), which is identified by mining expressed-sequence-tag databases, indicates that, in vivo, functional brain GABAB receptors may be heterodimers composed of GABABR1 and GABABR2.
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