Inhibition of HCV NS3 protease by RNA aptamers in cells.

  title={Inhibition of HCV NS3 protease by RNA aptamers in cells.},
  author={Fumiko Nishikawa and Nobuko Kakiuchi and Kohei Funaji and Kotaro Fukuda and Satoru Sekiya and S. Nishikawa},
  journal={Nucleic acids research},
  volume={31 7},
Non-structural protein 3 (NS3) of hepatitis C virus (HCV) has two distinct activities, protease and helicase, which are essential for HCV proliferation. In previous work, we obtained RNA aptamers (G9-I, II and III) which specifically bound the NS3 protease domain (DeltaNS3), efficiently inhibiting protease activity in vitro. To utilize these aptamers in vivo, we constructed a G9 aptamer expression system in cultured cells, using the cytomegarovirus enhancer + chicken beta-actin globin (CAG… CONTINUE READING


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