Inhibition of DNA-directed beta-galactosidase synthesis in a cell-free system by dimethyl sulfate and N-methyl-N-nitrosourea.

@article{Chen1980InhibitionOD,
  title={Inhibition of DNA-directed beta-galactosidase synthesis in a cell-free system by dimethyl sulfate and N-methyl-N-nitrosourea.},
  author={Benjamin P. Chen},
  journal={Carcinogenesis},
  year={1980},
  volume={1 5},
  pages={
          367-74
        }
}
Methylation of a genome DNA lambda h80dlacps by N-methyl-Nitrosourea (MNU, a potent carcinogen) and dimethyl sulfate (DMS, a weak carcinogen) results in loss of its template activity in directing beta-galactosidase (beta G) synthesis in vitro, the degree of inhibition in template activity is proportional to the extent of methylation which is in turn related to the concentration of the methylating agents during 10 minutes incubation at 37 degrees C. When these methylating agents are added to the… CONTINUE READING
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