Inhibition of Clostridium difficile strains by intestinal Lactobacillus species.

  title={Inhibition of Clostridium difficile strains by intestinal Lactobacillus species.},
  author={Paul Naaber and Imbi Smidt and Jelena {\vS}t{\vs}epetova and Tatjana Brilene and Heidi Annuk and Marika Mikelsaar},
  journal={Journal of medical microbiology},
  volume={53 Pt 6},
Indigenous intestinal microflora (including lactobacilli) has an important role in protection against Clostridium difficile infection. To assess in vitro interaction between lactobacilli and C. difficile, antagonistic activity of 50 intestinal Lactobacillus spp. strains against 23 pathogenic C. difficile strains was determined. Phenotypic properties of C. difficile strains [production of short-chain fatty acids (SCFAs) and toxin A, and antimicrobial susceptibility] and lactobacilli (production… 

Tables from this paper

Effect of Lactobacillus plantarum Strains on Clinical Isolates of Clostridium difficile in vitro

In this in vitro study, the influence of five Lactobacillus plantarum strains to the survival of C. difficile reference strains and clinical isolates was evaluated using co-culturing and micro-titre plate assay.

Lactic acid production by Streptococcus thermophilus alters Clostridium difficile infection and in vitro Toxin A production

In the context of infection with C. difficile, mice treated with viable S. thermophilus exhibited 46% less weight loss compared with untreated controls; moreover, less pathology, diarrhea, and lower detectable toxin levels in cecal contents were evident more often in S. therophillus treated mice.

Influence of the probiotic Escherichia coli strain Nissle 1917 on the growth of different pathogenic bacteria isolated from patients with diarrhea

In vitro growth inhibition was recorded in none of cultured bacterial samples, suggesting that the inhibitory activity of EcN on different bacteria probably relies on different in vivo complementary mechanisms.

Intestinal lacto fl ora in Estonian and Norwegian patients with antibiotic associated diarrhea

Results of total counts of lactobacilli comparing Estonian and Norwegian samples were conflicting by culture and PCR, indicating that in different persons and nations, different lactOBacilli species may have a protective role against C. difficile.

Bacteriocins of Probiotics and Enteric Cytoprotection

New possibilities such as the structural analysis, nuclear magnetic resonance, construction of recombinant probiotics with heterologous bacteriocin genes, protein-engineering used to synthesize modified bacteriOCins with improved properties, and increasing number of the whole genomes sequences of probiotic bacteria will enable to better identify and understand the relations between structure and function of bacteriaiocins.

Lactobacillus casei-fermented milk as an inhibitor on selected foodborne pathogens

The aim of this study was to reduce the growth of vanB resistant Enterococcus faecium, vanA resistant Enterococcus faecalis, Staphylococcus aureus ATCC 43300 and methicillin-resistant Staphylococcus

Characterization of the beneficial properties of lactobacilli isolated from bullfrog (Rana catesbeiana) hatchery

This paper constitute the first study related to the beneficial properties of Lactobacillus isolated from a bullfrog hatchery, as well as the selection criteria applied to a group of strains, which could help to control or prevent bacterial infectious diseases in raniculture.



[Antagonistic activity of Lactobacillus bacteria strains against anaerobic gastrointestinal tract pathogens (Helicobacter pylori, Campylobacter coli, Campylobacter jejuni, Clostridium difficile)].

All tested human Lactobacillus strains were able to inhibit the growth of all strains of anaerobic human gastrointestinal pathogens used in this study.

Clostridium difficile infection and intestinal microbial ecology

It is revealed that both xylitol and colostrum inhibited the adhesion of C. difficile to Caco-2 cells and the count of adherent C.difficile decreased when compared to the control.

Role of volatile fatty acids in colonization resistance to Clostridium difficile in gnotobiotic mice

It is suggested that VFAs alone cannot inhibit intestinal colonization by C. difficile and that, consequently, other inhibitory mechanisms are also present.

Antibacterial effect of the adhering human Lactobacillus acidophilus strain LB

The spent culture supernatant of the human Lactobacillus acidophilus strain LB produces an antibacterial activity against a wide range of gram-negative and gram-positive pathogens. It decreased the

Antagonistic action of Lactobacillus lactis toward Salmonella spp. and Escherichia coli O157:H7 during growth and refrigerated storage.

Interaction studies with pH-neutralized broth indicated that acid production by L lactis was primarily responsible for the inhibition of pathogens, and suggests that this strain of L. lactis does not inhibit Salmonella spp.

The effect of probiotics on Clostridium difficile diarrhea.

  • M. Pochapin
  • Medicine, Biology
    The American journal of gastroenterology
  • 2000

Antagonistic action of cells of Lactobacillus lactis toward Escherichia coli O157:H7 on refrigerated raw chicken meat.

Interaction experiments in the presence of catalase indicated that hydrogen peroxide was the main factor responsible for the inhibitory action produced by the lactobacilli.

Colonization of infants and hospitalized patients with Clostridium difficile and lactobacilli.

Both the prevalence and counts of C. difficile and intestinal lactobacilli were significantly higher among the Estonian infants than among the Swedish infants and neither of the 21 hospitalized patients was colonized by C.difficile during the first 2-month observation period, whereas seven of 13 patients were found to be colonized during the second period.

Isolation and characterization of two bacteriocins of Lactobacillus acidophilus LF221

Lactobacillus acidophilus LF221 produced bacteriocin-like activity against different bacteria including some pathogenic and food-spoilage species and may be of interest as a probiotic strain because of its human origin and inhibition of pathogenic bacteria, especially Clostridium difficile.

Prevalence of hydrogen peroxide-producing Lactobacillus species in normal women and women with bacterial vaginosis

The production of H2O2 by Lactobacillus species may represent a nonspecific antimicrobial defense mechanism of the normal vaginal ecosystem.