Inhibition of Aurora B Kinase Blocks Chromosome Segregation, Overrides the Spindle Checkpoint, and Perturbs Microtubule Dynamics in Mitosis

@article{Kallio2002InhibitionOA,
  title={Inhibition of Aurora B Kinase Blocks Chromosome Segregation, Overrides the Spindle Checkpoint, and Perturbs Microtubule Dynamics in Mitosis},
  author={Marko J. Kallio and M. Mccleland and P. Todd Stukenberg and Gary J. Gorbsky},
  journal={Current Biology},
  year={2002},
  volume={12},
  pages={900-905}
}

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References

SHOWING 1-10 OF 26 REFERENCES

Elevating the level of Cdc34/Ubc3 ubiquitin-conjugating enzyme in mitosis inhibits association of CENP-E with kinetochores and blocks the metaphase alignment of chromosomes

TLDR
These studies suggest that CENP-E targeting to kinetochores is regulated by ubiquitylation not involving proteasome-mediated degradation.

Drosophila Aurora B Kinase Is Required for Histone H3 Phosphorylation and Condensin Recruitment during Chromosome Condensation and to Organize the Central Spindle during Cytokinesis

TLDR
It is shown that depleting Aurora B kinase using double-stranded RNA interference in cultured Drosophila cells results in polyploidy, and conserved functions of Aurora B Kinase in chromosome transmission and cytokinesis are discussed.

Essential Roles of Drosophila Inner Centromere Protein (Incenp) and Aurora B in Histone H3 Phosphorylation, Metaphase Chromosome Alignment, Kinetochore Disjunction, and Chromosome Segregation

TLDR
It is revealed that INCENP is required for aurora B kinase function and confirmed that the chromosomal passengers have essential roles in mitosis.

CENP-E is essential for reliable bioriented spindle attachment, but chromosome alignment can be achieved via redundant mechanisms in mammalian cells.

TLDR
A model in which redundant mechanisms enable kinetochore microtubule binding and checkpoint monitoring in the absence of CENP-E is explained, but where reduced microtubules-binding efficiency, exacerbated by poor positioning at the spindle poles, results in chronically monooriented chromosomes and mitotic arrest.

AIR-2: An Aurora/Ipl1-related Protein Kinase Associated with Chromosomes and Midbody Microtubules Is Required for Polar Body Extrusion and Cytokinesis in Caenorhabditis elegans Embryos

TLDR
The results suggest that during each meiotic and mitotic division, AIR-2 may coordinate the congression of metaphase chromosomes with the subsequent events of polar body extrusion and cytokinesis.

Aurora B kinase exists in a complex with survivin and INCENP and its kinase activity is stimulated by survivin binding and phosphorylation.

TLDR
The data indicate that Aurora B kinase activity is regulated by both Survivin binding and cell cycle-dependent phosphorylation, and the hydrodynamic properties of the Aurora B/Survivin/INCENP complex are cell cycle regulated.

Mammalian p55CDC Mediates Association of the Spindle Checkpoint Protein Mad2 with the Cyclosome/Anaphase-promoting Complex, and is Involved in Regulating Anaphase Onset and Late Mitotic Events

TLDR
Investigation of the function of p55CDC in Saccharomyces cerevisiae and Drosophila and extracts prepared from M phase, but not from interphase HeLa cells, suggest that mammalian p55 CDC may be part of a regulatory and targeting complex for the anaphase-promoting complex.