Inhibition of AtMYB2 DNA-binding by nitric oxide involves cysteine S-nitrosylation.

@article{Serpa2007InhibitionOA,
  title={Inhibition of AtMYB2 DNA-binding by nitric oxide involves cysteine S-nitrosylation.},
  author={Viviane Isabel Serpa and Javier I Vernal and Lorenzo Lamattina and Erich Grotewold and Ra{\'u}l Oscar Cassia and Hern{\'a}n Terenzi},
  journal={Biochemical and biophysical research communications},
  year={2007},
  volume={361 4},
  pages={1048-53}
}
Nitric oxide (NO) can influence the transcriptional activity of a wide set of Arabidopsis genes. The aim of the present work was to investigate if NO modifies DNA-binding activity of AtMYB2 (a typical R2R3-MYB from Arabidopsis thaliana), by a posttranslational modification of its conserved Cys53 residue. We cloned a fully active minimal DNA-binding domain of AtMYB2 spanning residues 19-125, hereafter called M2D. In EMSA assays, M2D binds the core binding site 5'-[A]AACC[A]-3'. The NO donors SNP… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 36 extracted citations

Similar Papers

Loading similar papers…