Inhibition of 2A-mediated ‘cleavage’ of certain artificial polyproteins bearing N-terminal signal sequences

  title={Inhibition of 2A-mediated ‘cleavage’ of certain artificial polyproteins bearing N-terminal signal sequences},
  author={Pablo de Felipe and Garry A. Luke and Jeremy D. Brown and Martin D Ryan},
  booktitle={Biotechnology journal},
Where 2A oligopeptide sequences occur within ORFs, the formation of the glycyl-prolyl peptide bond at the C-terminus of (each) 2A does not occur. This property can be used to concatenate sequences encoding several proteins into a single ORF: each component of such an artificial polyprotein is generated as a discrete translation product. 2A and '2A-like' sequences have become widely utilised in biotechnology and biomedicine. Individual proteins may also be co- and post-translationally targeted… CONTINUE READING
28 Citations
61 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 28 extracted citations


Publications referenced by this paper.
Showing 1-10 of 61 references

Sitespecific release of nascent chains from ribosomes at a sense codon

  • V. A. Doronina, C. Wu, P. de Felipe, Sachs, S M.
  • Mol. Cell. Biol
  • 2008
Highly Influential
10 Excerpts

D.,Targeting of proteins derived from self-processing polyproteins containing multiple signal sequences

  • P. de Felipe, M. Ryan
  • Traffic 2004,
  • 2004
Highly Influential
8 Excerpts

Cotranslational, intraribosomal cleavage of polypeptides by the foot-and-mouth disease virus 2A peptide

  • P. de Felipe, L. E. Hughes, M. D. Ryan, J. D. Brown
  • J. Biol. Chem
  • 2003
Highly Influential
4 Excerpts

Similar Papers

Loading similar papers…