Inhibition by glutamate of phosphate-dependent glutaminase of rat kidney.

  title={Inhibition by glutamate of phosphate-dependent glutaminase of rat kidney.},
  author={Richard A. Shapiro and R F Morehouse and Norman P. Curthoys},
  journal={The Biochemical journal},
  volume={207 3},
A membrane-associated form of phosphate-dependent glutaminase was derived from sonicated mitochondria and purified essentially free of gamma-glutamyl transpeptidase activity. Increasing concentrations of phosphate cause a sigmoidal activation of the membrane-bound glutaminase. Phosphate also causes a similar effect on the rate of glutaminase inactivation by the two affinity labels, L-2-amino-4-oxo-5-chloropentanoic acid and 6-diazo-5-oxo-L-norleucine, as observed previously for the solubilized… CONTINUE READING


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