Inhibition by brefeldin A of protein secretion from the apical cell surface of Madin-Darby canine kidney cells.

@article{Low1991InhibitionBB,
  title={Inhibition by brefeldin A of protein secretion from the apical cell surface of Madin-Darby canine kidney cells.},
  author={Seng Hui Low and S H Wong and Bor Luen Tang and Peilin Tan and V. Nathan Subramaniam and Wan Jing Hong},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 27},
  pages={17729-32}
}
The effect of brefeldin A (BFA) on total and polarized protein secretion was examined in MDCK cells. Increasing concentrations of BFA have increasingly inhibitory effects on total protein secretion. The total protein secretion was essentially unaffected by BFA at 0.5 microgram/ml. When the BFA concentration was increased to 10 and 30 micrograms/ml, the total protein secretion was reduced to about 70 and 25%, respectively, of the control level. Consistent with this effect on total protein… CONTINUE READING