Inhibition by Anandamide and Synthetic Cannabimimetics of the Release of [3H]d-Aspartate and [3H]GABA from Synaptosomes Isolated from the Rat Hippocampus

  title={Inhibition by Anandamide and Synthetic Cannabimimetics of the Release of [3H]d-Aspartate and [3H]GABA from Synaptosomes Isolated from the Rat Hippocampus},
  author={Monia D'Amico and Carla Cannizzaro and Paolo Preziosi and Maria Martire},
  journal={Neurochemical Research},
Cannabinoids (CB) can act as retrograde synaptic mediators of depolarization-induced suppression of inhibition or excitation in hippocampus. This mechanism may underlie the impairment of some cognitive processes produced by these compounds, including short-term memory formation in the hippocampus. In this study, we investigated several compounds known to interact with CB receptors, evaluating their effects on K+-evoked release of [3H]d-aspartate ([3H]d-ASP) and [3H]GABA from superfused… 

Localization of the cannabinoid CB1 receptor and the 2-AG synthesizing (DAGLα) and degrading (MAGL, FAAH) enzymes in cells expressing the Ca2+-binding proteins calbindin, calretinin, and parvalbumin in the adult rat hippocampus

The identification of anatomically related-neuronal substrates that expressed 2-AG/CB1 signaling system and selective CaBPs should be considered when analyzing the cannabinoid signaling associated with hippocampal functions.

Regulation of Hippocampal Cannabinoid CB1 Receptor Actions by Adenosine A1 Receptors and Chronic Caffeine Administration: Implications for the Effects of Δ9-Tetrahydrocannabinol on Spatial Memory

A1 receptors exert a negative modulatory effect on CB1-mediated inhibition of GABA and glutamate release, and provides the first evidence of chronic caffeine-induced alterations on the cannabinoid system in the cortex and hippocampus, with functional implications in spatial memory.

Heteromer formation between cannabinoid type 1 and dopamine type 2 receptors is altered by combination cannabinoid and antipsychotic treatments

Clinical relevance of co‐exposure to cannabinoids and different antipsychotics over acute and prolonged time periods is highlighted as the differential effects of haloperidol and olanzapine also applied to γ‐aminobutyric acid release in STHdhQ7/Q7 cells and motor function in C57BL/6J male mice.



Cannabinoids inhibit the release of [3H]glutamate from rodent hippocampal synaptosomes via a novel CB1 receptor‐independent action

It is demonstrated that the action of cannabinoids on glutamate release in the hippocampus is pharmacologically distinct and independent from the cloned CB1 receptor.

Cannabinoid CB1 receptor-mediated inhibition of glutamate release from rat hippocampal synaptosomes.

  • Su-Jane Wang
  • Biology, Chemistry
    European journal of pharmacology
  • 2003

Sodium channel inhibition by anandamide and synthetic cannabimimetics in brain

Cannabis and the brain.

  • L. Iversen
  • Biology, Psychology
    Brain : a journal of neurology
  • 2003
Central effects of cannabinoids include disruption of psychomotor behaviour, short-term memory impairment, intoxication, stimulation of appetite, antinociceptive actions (particularly against pain of neuropathic origin) and anti-emetic effects.

Levels, metabolism, and pharmacological activity of anandamide in CB(1) cannabinoid receptor knockout mice: evidence for non-CB(1), non-CB(2) receptor-mediated actions of anandamide in mouse brain.

A ligand/receptor relationship between AEA and CB(1) in these two brain regions is suggested, where tonic activation of the receptor may tightly regulate the biosynthesis of its endogenous ligand.

Evidence for a new G protein-coupled cannabinoid receptor in mouse brain.

Assays of different central nervous system (CNS) regions demonstrated significant activity of cannabinoids in CB(1)(-/-) membranes from brain stem, cortex, hippocampus, diencephalon, midbrain, and spinal cord, but not basal ganglia or cerebellum.

Formation and inactivation of endogenous cannabinoid anandamide in central neurons

It is reported that anandamide is produced in and released from cultured brain neurons in a calcium ion-dependent manner when the neurons are stimulated with membrane-depolarizing agents, indicating that multiple biochemical pathways may participate in an andamide formation in brain tissue.

Levels, Metabolism, and Pharmacological Activity of Anandamide in CB1 Cannabinoid Receptor Knockout Mice

A ligand/receptor relationship between AEA and CB1 in these two brain regions, where tonic activation of the receptor may tightly regulate the biosynthesis of its endogenous ligand is suggested.

Cannabinoid Receptor Agonists Inhibit Glutamatergic Synaptic Transmission in Rat Hippocampal Cultures

Activation of cannabinoid receptors inhibits the presynaptic release of glutamate via an inhibitory G-protein in cultured rat hippocampal neurons, and cannabimimetics did not affect bicuculline-sensitive inhibitory postsynaptic currents.

A second endogenous cannabinoid that modulates long-term potentiation

2-AG activates neuronal cannabinoid receptors as a full agonist, and prevents the induction of long-term potentiation at CA3–CA1 synapses, indicating that 2-AG is a second endogenous cannabinoid ligand in the central nervous system.