Inhibition and enhancement of odorant-induced cAMP accumulation in rat olfactory cilia by antibodies directed against Gαs/olf- and Gαi-protein subunits

@inproceedings{Sinnarajah1998InhibitionAE,
  title={Inhibition and enhancement of odorant-induced cAMP accumulation in rat olfactory cilia by antibodies directed against Gαs/olf- and Gαi-protein subunits},
  author={Srikumar Sinnarajah and Patrick I. Ezeh and Suram Therese Pathirana and Anthony G. Moss and Edward Eugene Morrison and Vitaly Vodyanoy},
  year={1998}
}
The odorant-induced accumulation of cAMP can be inhibited by antibodies directed against Gαs/olf. In contrast, antibodies raised against Gαi-subunits caused a strong enhancement of the odorant-induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence of both GαS/olf- and Gαi-subunits in rat cilia preparations. The existence of both stimulatory and inhibitory odorant-induced regulation of adenylyl cyclase activity in olfactory cilia may indicate that an… CONTINUE READING