Inhibin: studies of stored and secreted forms by biosynthetic labeling and immunodetection in cultured rat granulosa cells.

@article{Bicsak1988InhibinSO,
  title={Inhibin: studies of stored and secreted forms by biosynthetic labeling and immunodetection in cultured rat granulosa cells.},
  author={Thomas A. Bicsak and Stefan B. Cajander and Wylie Vale and Aaron J Hsueh},
  journal={Endocrinology},
  year={1988},
  volume={122 2},
  pages={
          741-8
        }
}
The biosynthesis of inhibin in rat granulosa cells was studied by biosynthetic labeling, immunoblotting, and immunocytochemical techniques. Granulosa cells from immature hypophysectomized estrogen-treated rats were cultured in the presence of [35S]cysteine. Both conditioned media and cell extracts were subjected to immunoprecipitation with an antibody directed against the N-terminal 26 amino acids of the alpha-chain of porcine inhibin (pI alpha 1-26), followed by sodium dodecyl sulfate… CONTINUE READING

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Growth factors and their cellular actions.

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