Infrared studies of fully hydrated unsaturated phosphatidylserine bilayers. Effect of Li+ and Ca2+.

@article{Casal1987InfraredSO,
  title={Infrared studies of fully hydrated unsaturated phosphatidylserine bilayers. Effect of Li+ and Ca2+.},
  author={H{\'e}ctor L. Casal and A. Mart{\'i}n and Henry H. Mantsch and Fritz Paltauf and Helmut Hauser},
  journal={Biochemistry},
  year={1987},
  volume={26 23},
  pages={
          7395-401
        }
}
Infrared spectroscopy has been used to characterize the thermal-phase behavior of fully hydrated 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS) and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) as well as their interaction with Li+ and Ca2+. The order-disorder transition of POPS-NH4+ is at 17 degrees C; in the presence of Li+ a POPS-Li+ complex is formed, and the transition temperature of this complex is 40 degrees C. DOPS-NH4+ has an order-disorder transition at -11 degrees C… Expand
Infrared spectroscopic studies on the phosphatidylserine bilayer interacting with calcium ion: effect of cholesterol.
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One main conclusion of this work is that Mg2+ binding to PS bilayers shows a gradation, the binding is in the order DMPS greater than POPS greater than ox brain PS greater than DOPS. Expand
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High-pressure Fourier-transform infrared (FT-IR) spectroscopy was used to study the barotropic behavior of phosphatidylserine bilayers and their interactions with the local anesthetic tetracaine, showing that the barotrop behavior of the negatively charged phosphate bilayers is very similar to that observed for zwitterionic phospholipids, with corresponding acyl chains. Expand
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TLDR
The spectra support the hypothesis that several conformations are present in liquid crystalline phospholipid dispersions, and show that the C=O stretching band at 1741 cm-1 of phosphatidylserines previously assigned to the sn-1 C = O vibration contains contributions from both thesn-1 and theSn-2 carbonyls. Expand
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Analysis of the chemical shifts of the various lipid atoms under the differing ionic environments indicates that Ca2+ enhanced the deprotonation of both the carboxyl and amino groups and stabilized the entire polar head group against the effects of changing pH. Expand
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The X-ray diffraction data suggest that PS becomes dehydrated on complexing with Li+, Mg2+, Ca2+, and other divalent cations and adopts either the chain untilted (form I) or tilted (form II) bilayer structure. Expand
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