Infrared microspectroscopy study of gamma-irradiated and H2O2-treated human cells.

@article{Gault2005InfraredMS,
  title={Infrared microspectroscopy study of gamma-irradiated and H2O2-treated human cells.},
  author={Nathalie Gault and Odile Rigaud and J L Poncy and Jean-Louis Lefaix},
  journal={International journal of radiation biology},
  year={2005},
  volume={81 10},
  pages={767-79}
}
PURPOSE Fourier transform infrared microspectroscopy (FT-IRM), which allows simultaneous detection of biochemical changes in the various cellular compartments, was used as a new analytical tool to study early radiation- and oxidation-induced cellular damage at the molecular level in single human cells. MATERIALS AND METHODS HaCaT keratinocytes were given a single dose of 6 Gy (137Cs) or 650 microM H2O2, neither of which is cytotoxic (neutral red assay) but both of which result in less than 10… CONTINUE READING