Infrared and 31P-NMR studies of the effect of Li+ and Ca2+ on phosphatidylserines.

@article{Casal1987InfraredA3,
  title={Infrared and 31P-NMR studies of the effect of Li+ and Ca2+ on phosphatidylserines.},
  author={H. Casal and H. Mantsch and F. Paltauf and H. Hauser},
  journal={Biochimica et biophysica acta},
  year={1987},
  volume={919 3},
  pages={
          275-86
        }
}
Infrared and 31P-NMR spectra of solid samples of 1,2-dimyristoyl-sn-glycero-3-phospho-L-serine (DMPS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS) and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) have been recorded. Comparison of the spectra of the Na+ salts of these phospholipids with those of complexes formed with Li+ and Ca2+ ions allows the characterization of conformational changes induced by complexation with Li+ and Ca2+. Ca2+ forms tight, crystalline complexes with… Expand
Infrared and 31P-NMR studies of the interaction of Mg2+ with phosphatidylserines: effect of hydrocarbon chain unsaturation.
TLDR
One main conclusion of this work is that Mg2+ binding to PS bilayers shows a gradation, the binding is in the order DMPS greater than POPS greater than ox brain PS greater than DOPS. Expand
Infrared studies of fully hydrated unsaturated phosphatidylserine bilayers. Effect of Li+ and Ca2+.
TLDR
Infrared spectroscopy has been used to characterize the thermal-phase behavior of fully hydrated 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (POPS) and DOPS as well as their interaction with Li+ and Ca2+. Expand
Infrared spectroscopic studies on the phosphatidylserine bilayer interacting with calcium ion: effect of cholesterol.
TLDR
The IR data indicate that the presence of cholesterol induces disorder of the acyl chain packing, increases the degree of immobilization of the interfacial and polar regions, and increases the degrees of dehydration of the PS/Ca2+ complexes. Expand
Fourier transform infrared study of fully hydrated dimyristoylphosphatidylglycerol. Effects of Na+ on the sn-1' and sn-3' headgroup stereoisomers.
TLDR
It is demonstrated that Na+ binds tightly to 3'-DMPG, leading to the immobilization of the entire phospholipid polar headgroup, and thus reveals reduced motional freedom. Expand
Infrared spectroscopic study of the interaction of diacylglycerol with phosphatidylserine in the presence of calcium.
TLDR
The effects reported here for the interaction of DPG with DPPS may be significant for a number of biological situations where Ca2+, phosphatidylserine and diacylglycerols are involved, such as fusion of membranes or the activation of protein kinase C, where the dehydration effect produced by diacy lglycerol may explain, at least in part, their effects. Expand
A Fourier transform infrared spectroscopic study of the interaction of alkaline earth cations with the negatively charged phospholipid 1, 2-dimyristoyl-sn-glycero-3-phosphoglycerol.
The interaction of aqueous phospholipid dispersions of negatively charged 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol, sodium salt (DMPG) with the divalent cations Mg(2+), Ca(2+) and Sr(2+) atExpand
Conformation of phosphatidylserine in bilayers as studied by Fourier transform infrared spectroscopy.
TLDR
The spectra support the hypothesis that several conformations are present in liquid crystalline phospholipid dispersions, and show that the C=O stretching band at 1741 cm-1 of phosphatidylserines previously assigned to the sn-1 C = O vibration contains contributions from both thesn-1 and theSn-2 carbonyls. Expand
High-pressure infrared study of phosphatidylserine bilayers and their interactions with the local anesthetic tetracaine.
TLDR
High-pressure Fourier-transform infrared (FT-IR) spectroscopy was used to study the barotropic behavior of phosphatidylserine bilayers and their interactions with the local anesthetic tetracaine, showing that the barotrop behavior of the negatively charged phosphate bilayers is very similar to that observed for zwitterionic phospholipids, with corresponding acyl chains. Expand
Effect of sphingosine and stearylamine on the interaction of phosphatidylserine with calcium. A study using DSC, FT-IR and 45Ca(2+)-binding.
TLDR
Results show that SPH and SA, being positively charged molecules anchored in the membrane, are able of preventing the binding of positively charged ions such as Ca2+ through an electrostatic charge neutralization. Expand
Conformational effects of metal salt binding to the polar head of phosphatidylcholines investigated by FTIR spectroscopy
Abstract FTIR spectra of DPPC multibilayers with metal salts incorporated (EuCl3, Eu(NO3)3, UO2(NO3)2, CaCl2, Ca(NO3)2, MgCl2, NaCl, NaNO3, LiCl, LiNO3), dry and hydrated, were investigated withExpand
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References

SHOWING 1-10 OF 26 REFERENCES
Effect of water on the molecular structure of a phosphatidylcholine hydrate. Raman spectroscopic analysis of the phosphate, carbonyl and carbon-hydrogen stretching mode regions of 1,2-dipalmitoylphosphatidylcholine dihydrate.
TLDR
Spectra for crystalline DPPC diHydrate in the 3000 cm-1 C-H stretching mode region suggest a hybrid chain packing lattice which is analogous to the packing arrangement determined for the isostructurally similar DMPC dihydrate species. Expand
Effects of divalent cations and pH on phosphatidylserine model membranes: a 31P NMR study.
  • M. Hope, P. Cullis
  • Chemistry, Medicine
  • Biochemical and biophysical research communications
  • 1980
TLDR
The addition of Ca2+ results in the observation of a “rigid lattice” 31P-NMR spectra and more than an order of magnitude increase in the spin-lattice relaxation time T1, which corresponds to a strong and specific headgroup immobilization by Ca2+, similar to that observed for anhydrous phosphatidylserine. Expand
Crystallization of phosphatidylserine bilayers induced by lithium.
TLDR
It is raised the possibility that interaction of Li+ with anionic membrane phospholipids could play a role in its pharmacological action, in view of the widespread use of lithium in the treatment of manic-depressive illness. Expand
Binding of Ca2+ and Mg2+ to phosphatidylserine vesicles: different effects on P-31 NMR shifts and relaxation times.
Abstract Phosphorus-31 NMR lines corresponding to inner and outer surfaces of sonicated phosphatidylserine vesicles can be distinguished by the effects of added Ca 2+ or Mg 2+ at low bulkExpand
Differences in the interaction of inorganic and organic (hydrophobic) cations with phosphatidylserine membranes.
TLDR
It is shown that the reaction products (cation-phospholipid) differ markedly in their physicochemical behaviour and is consistent with NMR results which show that the phosphodiester group is involved in the binding of both classes of cations as well as being the site of the primary hydration shell. Expand
Structure and thermotropic behavior of phosphatidylserine bilayer membranes.
The structure and thermotropic properties of a homologous series of diacylphosphatidylserines (PS) in the anhydrous and hydrated states have been examined with low-angle X-ray diffraction andExpand
The thermotropic phase behavior of N-methylated dipalmitoylphosphatidylethanolamines
Abstract The thermal phase behavior of the mono- and di- N -methylated derivatives of DPPE has been investigated by DSC and infrared spectroscopy. It is shown that these partially N -methylated DPPEExpand
Crystalline anhydrous Ca-phosphatidylserine bilayers.
TLDR
The interaction of phosphatidylserine bilayers with Ca 2+ is equivalent to an isothermal transition of the bilayer from the liquid crystalline to the crystal state. Expand
Metastability and polymorphism in the gel phase of 1,2-dipalmitoyl-3-SN-phosphatidylcholine. A Fourier transform infrared study of the subtransition.
TLDR
It was found that when DPPC is incubated at 2 degrees C for three days the two-dimensional acyl chain packing changes from one resulting in spectra typical of an orthorhombic subcell to one resembling that found in triclinically packed acyl systems. Expand
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