Informatic and genomic analysis of melanocyte cDNA libraries as a resource for the study of melanocyte development and function.

@article{Baxter2007InformaticAG,
  title={Informatic and genomic analysis of melanocyte cDNA libraries as a resource for the study of melanocyte development and function.},
  author={Laura L Baxter and Benjamin J. Hsu and Lowell A. Umayam and Tyra G. Wolfsberg and Denise M. Larson and Martin C. Frith and Jun Kawai and Yoshihide Hayashizaki and Piero Carninci and William J. Pavan},
  journal={Pigment cell research},
  year={2007},
  volume={20 3},
  pages={
          201-9
        }
}
As part of the RIKEN mouse encyclopedia project, two cDNA libraries were prepared from melanocyte-derived cell lines, using techniques of full-length clone selection and subtraction/normalization to enrich for rare transcripts. End sequencing showed that these libraries display over 83% complete coding sequence at the 5' end and 96-97% complete coding sequence at the 3' end. Evaluation of the libraries, derived from B16F10Y tumor cells and melan-c cells, revealed that they contain clones for a… CONTINUE READING

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