Influencing in vitro clonal propagation of Chonemorpha fragrans (moon) Alston by culture media strength, plant growth regulators, carbon source and photo periodic incubation

  title={Influencing in vitro clonal propagation of Chonemorpha fragrans (moon) Alston by culture media strength, plant growth regulators, carbon source and photo periodic incubation},
  author={Tasiu Isah and Shahid Umar},
  journal={Journal of Forestry Research},
  • T. Isah, S. Umar
  • Published 18 September 2018
  • Biology
  • Journal of Forestry Research
Chonemorpha fragrans is an endangered medicinal woody climber, regarded among alternative plant sources of camptothecin. Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three billion U.S. dollars in the recent, and is used in the production of its analog drugs approved for the chemotherapy of cancer of varied types. Effects of plant growth regulators, culture media strength and photoperiodic duration on the micropropagation efficiency of C. fragrans… 

Influence of plant growth regulators on morphogenic response, biomass and camptothecin production in the callus cultures of Chonemorpha fragrans (Moon) Alston

  • T. Isah
  • Notulae Scientia Biologicae
  • 2019
Background: In vitro morphogenic response of mature seed embryo-derived callus cultures of Chonemorpha fragrans was studied using solid and liquid Murashige and Skoog medium amended with cytokinins

Proteome study of embryogenic competence acquisition in the callus cultures of Nothapodytes nimmoniana (J. Graham) Mabberly

The results of the study showed higher metabolic and physiological processes associated with embryogenic competence acquisition in the callus cultures; high cellular oxidative stress, energy metabolism, protein synthesis, and other metabolic processes played a key role in upregulated expression of the identified proteins in EC over NEC.

De novo in vitro shoot morphogenesis from shoot tip-induced callus cultures of Gymnema sylvestre (Retz.) R.Br. ex Sm

  • T. Isah
  • Medicine, Biology
    Biological Research
  • 2019
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Nodal segment explant type and preconditioning influence in vitro shoot morphogenesis in Ginkgo biloba L.

  • T. Isah
  • Biology
    Plant Physiology Reports
  • 2019
Results of the experiments showed that explants collection season influenced in vitro clonal propagation in G. biloba and the strategy employed in the study could be of application in theClonal propagation of non-responsive woody nodal segment explants(s) of other species.

Enhanced in vitro shoot proliferation through nodal explants of Staurogyne repens (Nees) Kuntze

In this study, multiple and rapid propagations of Staurogyne repens (Nees) Kuntze with tissue culture techniques were aimed and regenerated shoots for in vitro rooting were transferred to the growth culture containing different concentrations of IAA and 1-Naphthaleneacetic acid (NAA).

The impact of carbon source on cell growth and the production of bioactive compounds in cell suspensions of Hancornia speciosa Gomes

Belonging to the Brazilian flora, the species Hancornia speciosa (Gomes), known as mangabeira, has bioactive compounds of interest, such as flavonoids, xanthones, and proanthocyanidins. The objective

Leaf development and anatomy of in vitro-grown Polygala paniculata L. are affected by light quality, gelling agents, and sucrose

Plant in vitro growth performance is influenced by environmental, physical, and chemical conditions. The present study evaluated the mutual effect of light, gelling agents, and sucrose concentration

Proteome study of somatic embryogenesis in Nothapodytes nimmoniana (J. Graham) Mabberly

Analysis of the extracted proteins from globular, heart/torpedo-shaped, and maturing embryo stages resolved in the 2-DE gels showed increased protein expression across developmental stages of the somatic embryos.

Variation of Secondary Metabolite Contents and Activities against Bovine Diarrheal Pathogens among Zygophyllaceae Species in Benin and Implications for Conservation.

Balanites aegyptiaca is a wild plant species largely used in folk medicine and a priority fruit tree in West Africa. In Benin, its overexploitation for ethnoveterinary uses could lead to its rarity



Influence of Various Carbon Sources and Organic Additives on In Vitro Growth and Morphogenesis of Leptadenia reticulata (Wight & Arn), A Valuable Medicinal Plant of India

This study has derived a low cost protocol by using cheaper substitutes to medium for in vitro multiplication of L. reticulata on a large scale which is vital for tissue culture industries.

Carry-over effect of Thidiazuron on banana in vitro proliferation at different culture cycles and light incubation conditions.

Investigating the carry over effect of varying concentrations of TDZ and 22.2 mM benzylaminopurine as control on proliferation of five banana cultivars on a hormone free medium under various incubation conditions showed that TDZ had a carry-over effect that enabled shoots to continue proliferating on a hormones free medium as the culture cycles increased and that this effect was significantly higher than that of BAP.

Effects of flask sealing and growth regulators on in vitro propagation of neem (Azadirachta indica A. Juss.)

The results indicated that the use of flasks with vents was beneficial for in vitro propagation of this important plant, and explants cultured in flasks without membranes showed leaf chlorosis and senescence.

In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered medicinal plant

In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered species of Stemonaceae, was established and regenerated plantlets were successfully transferred to soil.

In vitro growth of Brassocattleya orchid hybrid in different concentrations of KNO3, NH4NO3 and benzylaminopurine

One of the most important applications of plant tissue culture is mass propagation of ornamental plants. This experiment evaluated the effect of different concentrations of NH 4 NO 3 and KNO 3 and

A modified Murashige and Skoog media for efficient multipleshoot induction in G. arborea Roxb.

This study reports on the effect of various micropropagation factors of Gmelina arborea Roxb through multiple shoot induction and recommends the use of glucose in place of sucrose in MS medium for maximum regeneration efficiency.

Thidiazuron: a potent cytokinin for woody plant tissue culture

Thidizuron (TDZ) is among the most active cytokinin-like substances for woody plant tissue culture. It facilitates efficient micropropagation of many recalcitrant woody species. Low concentrations

TDZ pulsing evaluation on the in vitro morphogenesis of peach palm

To asses TDZ pulsing effect on B. gasipaes morphogenesis, explants obtained from unarmed microplants were maintained in two treatments, half of them in MS free medium (without growth regulator) and the other half in MS with TDZ (0.36 μM).

Photoperiod and plant growth regulator combinations influence growth and physiological responses in Pelargonium sidoides DC.

Combinations of MemTR and auxins had a synergistic effect on the concentration of photosynthetic pigments and total phenolic compounds and flavonoids and had a significant effect onThe influence of photoperiod and meta-methoxytopolin riboside (MemTR)-auxin combinations on in vitro growth and physiological responses of Pelargonium sidoides DC was evaluated.

Influence of Plant Growth Regulators on in Vitro Seed Germination and Seedling Development of Digitalis purpurea L .

Digitalis purpurea L. (foxglove; Scrophulariaceae) is an herbaceous medicinally important cardiac glycoside-producing plant. The aim of the present study was to access the seed viability and