Influences of acidic conditions on formazan assay: a cautionary note.

@article{Johno2010InfluencesOA,
  title={Influences of acidic conditions on formazan assay: a cautionary note.},
  author={Hisashi Johno and Shuhei Takahashi and Masanori Kitamura},
  journal={Applied biochemistry and biotechnology},
  year={2010},
  volume={162 6},
  pages={1529-35}
}
Formazan assay has been used for several decades to evaluate metabolic activity of eukaryotic and prokaryotic cells. In particular, it has been often applied for quantitative assessment of viable cells under acidic circumstances caused by, e.g., ischemia and hypoxia. However, little attention has been paid to the influence of acidic pH on formazan assays. We found that acidic culture conditions significantly affect outcomes of the assays. Absorbance of tetrazolium-formazan decreased in a pH… CONTINUE READING

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