Influence of viral genes on the cell-to-cell spread of RNA silencing

  title={Influence of viral genes on the cell-to-cell spread of RNA silencing},
  author={Yu Zhou and Eugene V Ryabov and Xuemei Zhang and Yiguo Hong},
  journal={Journal of Experimental Botany},
  pages={2803 - 2813}
The turnip crinkle virus-based vector TCV-GFP Delta CP had been devised previously to study cell-to-cell and long-distance spread of virus-induced RNA silencing. TCV-GFP Delta CP, which had been constructed by replacing the coat protein (CP) gene with a green fluorescent protein (GFP) coding sequence, was able to induce RNA silencing in single epidermal cells, from which RNA silencing spread from cell-to-cell. Using this unique local silencing assay together with mutagenesis analysis, two TCV… CONTINUE READING
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