Influence of the ion-composition of the medium on alkaloid production by “hairy roots” ofDatura stramonium

  title={Influence of the ion-composition of the medium on alkaloid production by “hairy roots” ofDatura stramonium},
  author={N. N. Sikuli and Kimberly Demeyer},
  journal={Plant Cell, Tissue and Organ Culture},
The influence of culture age on biomass production and alkaloid yield of “hairy roots” obtained after infection ofDatura stramonium L. withAgrobacterium ATCC 15834 was investigated. Maximal hyoscyamine yield was obtained with roots harvested after six weeks. Fluctuations were found for tropine yield, the precursor of the ring moiety of hyoscyamine. These indicate a continuous conversion to hyoscyamine during the exponential growth phase. The effect of the ion-balance was investigated by… CONTINUE READING


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