Influence of temperature and sperm preparation on the quality of spermatozoa.

@article{Thijssen2014InfluenceOT,
  title={Influence of temperature and sperm preparation on the quality of spermatozoa.},
  author={Annelies Thijssen and Elke Klerkx and Carin Huyser and Eugene Bosmans and R. L. Campo and Willem U A M Ombelet},
  journal={Reproductive biomedicine online},
  year={2014},
  volume={28 4},
  pages={436-42}
}
This study investigated the effects of long-term (24h) in-vitro sperm incubation at room temperature (RT; 23°C) versus testis temperature (35°C) on various sperm-quality parameters. Semen samples (n=41) were prepared both by density-gradient centrifugation (DGC) and the swim-up technique in order to compare the influence of sperm preparation on sperm quality after incubation. Progressive motility and morphology were significantly higher after incubation at RT compared with 35°C (P<0.001 and P<0… CONTINUE READING