Influence of sulphate groups in the binding of peanut agglutinin. Histochemical demonstration with ligh- and electron-microscopy

@article{MartnezMenrguez1992InfluenceOS,
  title={Influence of sulphate groups in the binding of peanut agglutinin. Histochemical demonstration with ligh- and electron-microscopy},
  author={Jos{\'e} A Mart{\'i}nez-Men{\'a}rguez and Jos{\'e} Ballesta and Manuel Avil{\'e}s and Juan Francisco Madrid and Mar{\'i}a Teresa Castells},
  journal={The Histochemical Journal},
  year={1992},
  volume={24},
  pages={207-216}
}
The influence of sulphation of mucus glycoproteins in the binding of peanut agglutinin (PNA) to tissue sections has been investigated by means of histochemical techniques at the light- and electron-microscopic level. A sequential methylation-saponification procedure was applied for the desulphation of tissue samples. Labelling by peroxidase- and colloidal gold-conjugated PNA was compared in control and desulphated samples of rat intestinal mucosa. The high-iron-diamine (HID) technique was used… CONTINUE READING

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