Influence of substratum wettability on the strength of adhesion of human fibroblasts.

@article{Kooten1992InfluenceOS,
  title={Influence of substratum wettability on the strength of adhesion of human fibroblasts.},
  author={Theo G. van Kooten and J. M. Schakenraad and Henny C van der Mei and Henk J. Busscher},
  journal={Biomaterials},
  year={1992},
  volume={13 13},
  pages={897-904}
}
To determine the strength of adhesion and the detachment mechanisms of fibroblasts from substrata with different wettability, the behaviour of adhered cells was studied in a parallel-plate flow chamber during exposure to shear. Adhered cells were observed in situ, i.e. in the flow chamber, by phase-contrast microscope and images were analysed semiautomatically. Detachment was found to be dependent both on shear stress and time, although a critical shear stress can be found below which no… CONTINUE READING