Influence of stromal cells on lymphocyte adhesion and migration on endothelial cells.

@article{McGettrick2010InfluenceOS,
  title={Influence of stromal cells on lymphocyte adhesion and migration on endothelial cells.},
  author={Helen M McGettrick and Chris D. Buckley and G Ed Rainger and Gerard B Nash},
  journal={Methods in molecular biology},
  year={2010},
  volume={616},
  pages={49-68}
}
Methods are described for analysing adhesion and migration of isolated lymphocytes on endothelial cell monolayers which have been co-cultured with different stromal cells, with or without additional cytokine treatment. The different cells types are grown on opposite sides of 3.0- or 0.4-mum pore filters depending on whether migration through the whole construct is to be analysed or adhesion to the endothelial cells alone. Assays may be "static" or filters can be incorporated into flow chambers… CONTINUE READING

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