Influence of storage container for frozen-thawed chicken semen and frequency of insemination on fertility and its duration.

@article{Latorre1988InfluenceOS,
  title={Influence of storage container for frozen-thawed chicken semen and frequency of insemination on fertility and its duration.},
  author={J R Latorre and Guillermina Bautista Harris and Zelpha B. Johnson},
  journal={Poultry science},
  year={1988},
  volume={67 2},
  pages={333-5}
}
Pooled semen was diluted with Lake's solution containing 10.8% (v/v) glycerol. Aliquots of 1.2 mL and .5 mL were transferred to 1.8-mL capacity plastic vials and .5-mL capacity plastic straws, respectively. Semen was processed at 5 C, frozen by cooling at a rate of 3 C/min to -35 C, then plunged in liquid nitrogen. Samples were thawed at 5 C, diluted stepwise, centrifuged, and resuspended with nonglycerolized diluent at 5 C. Fertility and its duration were measured after single or double… CONTINUE READING