Influence of specific regions in Lp82 calpain on protein stability, activity, and localization within lens.

@article{Ma2000InfluenceOS,
  title={Influence of specific regions in Lp82 calpain on protein stability, activity, and localization within lens.},
  author={Hong Ma and Marjorie Shih and Chiho Fukiage and Mitsuyoshi Azuma and Melinda K. Duncan and Nathan A. Reed and I. Richard and Jacques S. Beckmann and Thomas R. Shearer},
  journal={Investigative ophthalmology & visual science},
  year={2000},
  volume={41 13},
  pages={4232-9}
}
PURPOSE To determine the influence of specific regions within Lp82 calpain on protein stability, enzymatic activity, and localization within lens and to test the influence of an Lp82 knockout mouse on normal maturational proteolysis in lens. METHODS DNA constructs for Lp82 and Lp82-related proteins were subcloned into the pcDNA 3.1 vector. The constructs… CONTINUE READING