Influence of single amino acids on the development of hamster one-cell embryos in vitro.

@article{Bavister1990InfluenceOS,
  title={Influence of single amino acids on the development of hamster one-cell embryos in vitro.},
  author={Barry D. Bavister and T Arlotto},
  journal={Molecular reproduction and development},
  year={1990},
  volume={25 1},
  pages={45-51}
}
One-cell hamster embryos placed in culture have always shown a complete block to development at the two-cell stage. In a preliminary study using a chemically defined culture medium containing 20 amino acids (HECM-1), many one-cell embryos were able to escape the "two-cell block" and develop to the four-cell stage. Use of a simpler formulation containing only the amino acids hypotaurine and glutamine revealed marked inhibitory and stimulatory effects of adding the other amino acids. In the first… CONTINUE READING
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