Influence of phospholipid saturation on classical thin-layer chromatographic detection methods and its effect on amniotic fluid lecithin/sphingomyelin ratio determinations.

@article{Spillman1983InfluenceOP,
  title={Influence of phospholipid saturation on classical thin-layer chromatographic detection methods and its effect on amniotic fluid lecithin/sphingomyelin ratio determinations.},
  author={Thomas W. Spillman and David B. Cotton and Stu Lynn and J. P. Bretaudiere},
  journal={Clinical chemistry},
  year={1983},
  volume={29 2},
  pages={
          250-5
        }
}
We compared seven techniques, commonly used for detection of amniotic fluid phospholipids in thin-layer chromatography, with respect to their sensitivity to saturation of the fatty acid carbon-chain of lecithin. The techniques fell into two classes: sensitive and insensitive; those classed as saturation sensitive were less than or equal to 10% as sensitive to fully saturated lecithin as to lecithin with singly unsaturated acid moieties. Color development increased with the number of carbon… CONTINUE READING

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