Influence of oocyte collection technique on initial chromatin configuration, meiotic competence, and male pronucleus formation after intracytoplasmic sperm injection (ICSI) of equine oocytes.

@article{Dellaquila2001InfluenceOO,
  title={Influence of oocyte collection technique on initial chromatin configuration, meiotic competence, and male pronucleus formation after intracytoplasmic sperm injection (ICSI) of equine oocytes.},
  author={Maria Elena Dell'aquila and Margaret Masterson and Filippo Maritato and Katrin Hinrichs},
  journal={Molecular reproduction and development},
  year={2001},
  volume={60 1},
  pages={79-88}
}
There is a great variability in the success of horse oocyte maturation and fertilization among laboratories. This study was conducted to determine if the meiotic and developmental competence of horse oocytes could be dependent on the method of oocyte collection, i.e., aspiration of follicular fluid with a vacuum apparatus, or opening follicles and scraping the granulosa layer. Horse oocytes were recovered from abattoir ovaries by aspiration or scraping and classified as having compact (Cp… CONTINUE READING

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