Influence of low temperature on productivity, proteome and protein phosphorylation of CHO cells.

@article{Kaufmann1999InfluenceOL,
  title={Influence of low temperature on productivity, proteome and protein phosphorylation of CHO cells.},
  author={Hitto Kaufmann and Xenia Mazur and Martin Fussenegger and J. E. Bailey},
  journal={Biotechnology and bioengineering},
  year={1999},
  volume={63 5},
  pages={573-82}
}
Proliferation of mammalian cells can be controlled by low cultivation temperature. However, depending on cell type and expression system, varying effects of a temperature shift on heterologous protein production have been reported. Here, we characterize growth behavior and productivity of the Chinese hamster ovary (CHO) cell line XM111-10 engineered to synthesize the model-product-secreted alkaline phosphatase (SEAP). Shift of cultivation temperature from 37 degrees C to 30 degrees C caused a… CONTINUE READING

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