Influence of extracellular matrix proteins on human keratinocyte attachment, proliferation and transfer to a dermal wound model.

@article{Dawson1996InfluenceOE,
  title={Influence of extracellular matrix proteins on human keratinocyte attachment, proliferation and transfer to a dermal wound model.},
  author={Rebecca A. Dawson and N J Goberdhan and Eric Freedlander and Sheila MacNeil},
  journal={Burns : journal of the International Society for Burn Injuries},
  year={1996},
  volume={22 2},
  pages={93-100}
}
The aim of this study was to investigate whether prior culture of cells on ECM proteins might positively influence the performance of keratinocytes when cells are transferred to a dermal in vitro wound bed model. Keratinocytes were cultured using a method for producing cultured epithelial autografts for severely burned patients (essentially using Green's medium, a mitogen-rich medium containing fetal calf serum, cholera toxin, EGF, insulin, transferrin and triiodothyronine). Cells were cultured… CONTINUE READING