Influence of dry heat treatment and anticoagulant on coupling of fibrinogen and fibronectin to von Willebrand factor in normal plasma.

@article{Ellegrd1992InfluenceOD,
  title={Influence of dry heat treatment and anticoagulant on coupling of fibrinogen and fibronectin to von Willebrand factor in normal plasma.},
  author={K H Elleg{\aa}rd and Kirsten Christiansen and J\orgen Ingerslev},
  journal={Scandinavian journal of clinical and laboratory investigation},
  year={1992},
  volume={52 7},
  pages={731-9}
}
In plasma from healthy subjects a coupling was identified between von Willebrand factor (vWf), fibrinogen (fg), and fibronectin (fn) that was dependent of anticoagulants heparin, EDTA, and citrate. Binding was quantitated by ELISA methodologies, based on polyclonal antibodies directed against the proteins studied, in order to express the percentage of moles of fg or fn bound to moles vWf, C[fg/vWf] or C[fn/vWf] (mol/mol)%. The intra-assay coefficients of variation (CV%) for fg and fn bound to… CONTINUE READING