Influence of cultural and physiochemical factors on ascorbate stability in plant tissue culture media

  title={Influence of cultural and physiochemical factors on ascorbate stability in plant tissue culture media},
  author={H. Wayne Elmore and Barbara Samples and Sanjeev Sharma and Marcia A. Harrison},
  journal={Plant Cell, Tissue and Organ Culture},
Ascorbic acid rapidly decays in plant tissue culture media. Within 50 min to 3 h after preparing 100 mM solutions, ascorbic acid was destroyed. Autoclaving, shaking flasks, high light intensity and increasing pH over a range from 4.5–7 accelerated decay. Ascorbic acid was oxidized to dehydroascorbic acid which also underwent decay. Within 11 h and 15 min after adding ascorbic acid both ascorbic acid and its oxidation product, dehydroascorbic acid, disappeared from medium. Since ascorbic acid is… CONTINUE READING


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