Influence of cultivation conditions on the production of staphylocoagulase by Staphylococcus aureus 104.

@article{Engels1978InfluenceOC,
  title={Influence of cultivation conditions on the production of staphylocoagulase by Staphylococcus aureus 104.},
  author={Wim J.M. Engels and Miriam A.F. Kamps and Cees P. A. van Boven},
  journal={Journal of general microbiology},
  year={1978},
  volume={109 2},
  pages={237-43}
}
High yields of staphylocoagulase from Staphylococcus aureus 104 were obtained in a simple salts medium supplement with glycerol, casein hydrolysate and three vitamins. Conditions of oxygen-limitation (dissolved oxygen concentration less than 2%), a pH of 7.4, a temperature of 35 degrees C and a 1 in 10 inoculum of overnight culture were required for optimal yields of staphylocoagulase. 

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