Influence of colchicine treatment on chromosome number and growth rate of tissue cultures of Valeriana wallichii DC

Cell cultures of Valeriana wallichii were treated with 0.05%, 0.2% and 0.5% of colchicine. The treatment with 0.05% and 0.2% colchicine resulted in well growing cultures. At the highest dose the cells died. The colchicine treatment could be repeated after six alkaloid free passages. The chromosome numbers shifted to polyploidy (n>96) under the treatment but… CONTINUE READING