Influence of chondrocytes on the chondrogenic differentiation of adipose stem cells.

@article{Lee2010InfluenceOC,
  title={Influence of chondrocytes on the chondrogenic differentiation of adipose stem cells.},
  author={J H Lee and Gun-Il Im},
  journal={Tissue engineering. Part A},
  year={2010},
  volume={16 12},
  pages={3569-77}
}
In this study, whether or not chondrogenic differentiation of adipose-derived stem cells (ASCs) could be enhanced by soluble factors from or coculture with chondrocytes was determined. In vitro pellet cultures were carried out in five ways using ASCs or chondrocytes in passage 3 as follows: #1, 2.5 × 10⁵ ASCs were cultured in Dulbecco's modified Eagle's medium/F-12 supplemented with 1% ITS, 10⁻⁷ M dexamethasone, 50 μM ascorbate-2-phosphate, 50 μM L-proline, and 1 mM sodium pyruvate; #2, 2.5… CONTINUE READING
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