Influence of carrageenan on cytokine production and cellular activity of mouse peritoneal macrophages and its effect on experimental endotoxemia.

  title={Influence of carrageenan on cytokine production and cellular activity of mouse peritoneal macrophages and its effect on experimental endotoxemia.},
  author={Aleksandra A. Kalitnik and Yu Karetin and Anna O. Kravchenko and Eleonora Izrailevna Khasina and Irina M. Yermak},
  journal={Journal of biomedical materials research. Part A},
  volume={105 5},
The in vivo effect of κ/β-carrageenan isolated from the red alga Tichocarpus crinitus on cytokine synthesis and cellular activity of murine peritoneal macrophages and also the protective effect of polysaccharides in LPS-induced endotoxemia in mice was studied. It was established that κ/β-carrageenan given orally at a dose of 100 mg/kg stimulates the induction of anti-inflammatory cytokines (IL-10) in mouse blood cells by more than 2.5-fold compared with control, with no effect on pro… 

Inhibitory Effects of Carrageenans on Endotoxin-Induced Inflammation

The results allow us to hope for the practical application of CRGs for lowering the endotoxemia level in patients under the development of the infectious process caused by Gram-negative bacteria.

Immunomodulating Properties of Carrageenan from Tichocarpus crinitus

It is demonstrated that κ/β-carrageenan, when administered orally, is not only not immunologically inert, but at the dose of 100 mg/kg possesses pharmacologically exploitable effects.

Immunological effects of Chondrusarmatus carrageenans and their low molecular weight degradation products.

It is shown that κ- and λ-carrageenans of the C. armatus and their LMWDPs suppress phagocytotic activity of peritoneal macrophages under both in vitro and in vivo conditions, which allows them to be viewed as pharmacologically active substances and justifies the need for their further investigation as such.

In Vitro antitumor and immunotropic activity of carrageenans from red algae Chondrus armatus and their low molecular weight degradation products.

It is demonstrated that LMW carrageenan degradation products not only retain biological activity of their precursors, but also increase their efficacy in type-dependent manner, allowing for their future development for pharmacological practice.

A carrageenan/agarose composite sponge and its immunomodulatory activities towards RAW264.7.

Results of particle size and intracellular concentration analyses suggested that the released carrageenan might enter into the cellular interior of RAW264.7 in the form of microgels or protein complexes.

Macroalgal dietary glycans: potential source for human gut bacteria and enhancing immune system for better health

Review focuses on the recent development of mechanistic understanding of human gut bacterial communities for utilizing macroalgal derived glycans and trends of application of glycans in modulating immune system at mechanistic level.

Multiparametric comparative analysis of coelomocytes in Asterias amurensis and Lysastrosoma anthosticta

Behavioral dynamics of coelomocytes from echinoderms Asterias amurensis and Lysastrosoma anthosticta during the first hour of in vitro cultivation was analyzed using a wide range of linear and fractal parameters of the external morphology, showing species-specific behavior of the immune cells of the studied animals.



Effects of carrageenan on cell permeability, cytotoxicity, and cytokine gene expression in human intestinal and hepatic cell lines.

Carrageenans effect on neutrophils alone and in combination with LPS in vitro.

Influence of sulfated red algal polysaccharides (κ-, λ-, and κ/β-carrageenans) and degraded derivative of κ/β-carrageenan on neutrophils/monocytes activation alone and in combination with

Influence of red algal polysaccharides on biological activities and supramolecular structure of bacterial lipopolysaccharide

Kappa/beta carrageenan was able to increase the synthesis of anti-inflammatory interleukin-10 (IL-10) in vitro, and at low concentrations, their activity in the mixture with LPS was higher than that of LPS alone.

Effects of IL-10 on systemic inflammatory responses during sublethal primate endotoxemia.

The results suggest that during sublethal endotoxemia the predominant anti-inflammatory effect of IL-10 treatment is inhibition of proinflammatory cytokine release.

Inhibition of Lipopolysaccharide (LPS)-induced inflammatory responses by Sargassum hemiphyllum sulfated polysaccharide extract in RAW 264.7 macrophage cells.

The secretion profiles of pro-inflammatory cytokines were found significantly to be reduced in 1-5 mg/mL dose ranges of SHSP treatments, and RT-PCR analysis suggested SHSP inhibits the LPS-induced mRNA expressions of IL-β, iNOS, and COX-2 in a dose-dependent manner.

Interleukin 10 protects mice from lethal endotoxemia

The protective effect of IL-10 was reversed by prior injection of neutralizing anti-IL-10 antibodies, and correlated with a substantial decrease in endotoxin-induced TNF-alpha release, which implicate IL- 10 as a candidate for treatment of bacterial sepsis, and more generally as an effective antiinflammatory reagent.

Oligosaccharides of κ/β-carrageenan from the red alga Tichocarpus crinitus and their ability to induce interleukin 10

The structural peculiarities of oligosaccharide mixture (LMW-carr) obtained by mild acid hydrolysis of κ/β-carrageenan from the red alga Tichocarpus crinitus were investigated by negative-ion

Attenuation of proinflammatory response by recombinant human IL-10 in human endotoxemia: effect of timing of recombinant human IL-10 administration.

It is indicated that pretreatment with rhIL-10 reduces endotoxin-induced febrile responses, cytokine responses, and granulocyte accumulation in lungs, while in this acute model post-treatment with rh IL-10 exerts limited anti-inflammatory effects.