Influence of alterations in culture condition and changes in perfusion parameters on the retention performance of a 20 μm spinfilter during a perfusion cultivation of a recombinant CHO cell line in pilot scale

@article{Iding2000InfluenceOA,
  title={Influence of alterations in culture condition and changes in perfusion parameters on the retention performance of a 20 μm spinfilter during a perfusion cultivation of a recombinant CHO cell line in pilot scale},
  author={Kai Iding and Dirk L{\"u}tkemeyer and Elisabeth Fraune and Kurt Gerlach and Juergen Lehmann},
  journal={Cytotechnology},
  year={2000},
  volume={34},
  pages={141-150}
}
Since 1969 much attention has been devoted to the useof spinfilter systems for retention of mammalian cellsin continuous perfusion cultivations. Previousinvestigations dealt with hydrodynamic conditions,fouling processes and upscaling. But hydrodynamicconditions and fouling processes seem to have asecondary importance in spinfilter performance duringauthentic perfusion cultivations. Obviously,alterations in culture condition are more relevantespecially during long-term processes. Therefore… CONTINUE READING
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