Influence of Substituent Chain Branching on the Transfection Efficacy of Cyclopropenium-Based Polymers

@article{Brucks2017InfluenceOS,
  title={Influence of Substituent Chain Branching on the Transfection Efficacy of Cyclopropenium-Based Polymers},
  author={Spencer D. Brucks and Jessica L. Freyer and Tristan H. Lambert and Luis M. Campos},
  journal={Polymers},
  year={2017},
  volume={9}
}
The realization of gene therapy relies on the development of delivery vectors with high efficiency and biocompatibility. With a multitude of structures accessible, the core challenge is precisely tuning vector structure to probe and optimize structure–property relationships. Employing a modular strategy, two pairs of cationic polymers based on the trisaminocyclopropenium (TAC) ion were synthesized where the substituents differ in the degree of alkyl chain branching. All TAC-based polymers… 

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References

SHOWING 1-10 OF 28 REFERENCES

A Single Methylene Group in Oligoalkylamine-Based Cationic Polymers and Lipids Promotes Enhanced mRNA Delivery.

Findings reveal a novel fundamental structure-activity relationship for the delivery of mRNA that is independent of the class of mRNA carrier and define a promising new path of exploration in the field of mRNA therapeutics.

Functionalization of poly(amidoamine) dendrimers with hydrophobic chains for improved gene delivery in mesenchymal stem cells.

Clickable Poly(ionic liquids): A Materials Platform for Transfection.

A post-polymerization click reaction is reported that provides facile access to trisaminocyclopropenium (TAC) ion-functionalized macromolecules of various architectures, which are the first class of polyelectrolytes that bear a formal charge on carbon.

Linear cationic click polymer for gene delivery: synthesis, biocompatibility, and in vitro transfection.

Two polymers showed good biocompatibility, inducing neither apoptosis nor necrosis at the test concentration and low hemolysis ratio and erythrocyte aggregation, and suggested that the novel cationic CPs could be promising carriers for gene delivery.

The transition from linear to highly branched poly(β-amino ester)s: Branching matters for gene delivery

The findings prove that the A2 + B3 + C2 approach is highly generalizable and flexible for the design and synthesis of HPAEs, which cannot be achieved by the conventional polymerization approach; H PAEs are more efficient vectors in gene transfection than the corresponding LPAEs.

Pentalysine-grafted ROMP polymers for DNA complexation and delivery.

Of particular interest was the excellent cell viability seen in experiments with polyplexes formed from the pentalysine-grafted polymers, the highest molecular weight graft copolymer, polymer 1e.

Biodegradable Highly Branched Poly(β-Amino Ester)s for Targeted Cancer Cell Gene Transfection.

Results show that because of the triggered degradability of the vector and enhanced receptor-mediated cellular uptake of polyplexes, the HPAESS-FA and HPAess-Lac exhibit superior gene transfection capability in specific cancer cells with negligible cytotoxicity, pointing to their promise as targeted vectors for efficient cancer gene therapy.

Influence of Polyplex Formation on the Performance of Star-Shaped Polycationic Transfection Agents for Mammalian Cells

Four star-shaped polycationic structures, all based on (2-dimethylamino) ethyl methacrylate (DMAEMA) building blocks, were investigated for their potential to deliver DNA to adherent and non-adherent mammalian cells and slightly outperformed the covalently linked agents.

Influence of polycation molecular weight on poly(2-dimethylaminoethyl methacrylate)-mediated DNA delivery in vitro.

The molecular weight of PDMAEMA was found to have a dramatic influence on transfection efficiency, and luciferase reporter gene expression increased as a function of increasing molecular weight, which suggested that the intracellular fate of the polyplexes is more important to overall transfections efficiency than barriers to entry, such as polyplex size.

Influence of polymer architecture and molecular weight of poly(2-(dimethylamino)ethyl methacrylate) polycations on transfection efficiency and cell viability in gene delivery.

It is proposed that polymers with a branched architecture and an intermediate molecular weight are the most promising candidates for efficient gene delivery, since they combine low cytotoxicity with acceptable transfection results.