Influence of Substituent Chain Branching on the Transfection Efficacy of Cyclopropenium-Based Polymers

  title={Influence of Substituent Chain Branching on the Transfection Efficacy of Cyclopropenium-Based Polymers},
  author={Spencer D. Brucks and Jessica L. Freyer and Tristan H. Lambert and Luis M. Campos},
The realization of gene therapy relies on the development of delivery vectors with high efficiency and biocompatibility. With a multitude of structures accessible, the core challenge is precisely tuning vector structure to probe and optimize structure–property relationships. Employing a modular strategy, two pairs of cationic polymers based on the trisaminocyclopropenium (TAC) ion were synthesized where the substituents differ in the degree of alkyl chain branching. All TAC-based polymers… Expand
7 Citations
Cyclopropenium Nanoparticles and Gene Transfection in Cells
This article introduces for the first time, cyclopropenium-containing nanoparticles as a cationic carrier for gene transfection, as an alternative to the common quaternary ammonium transfections agents. Expand
Synthesis and Characterization of Segmented Polyurethanes Containing Trisaminocyclopropenium Carbocations
Diol-functionalized trisaminocyclopropenium (TACP) carbocations were used as chain extenders in a two-step synthesis of a segmented polyurethane. Differential scanning calorimetry demonstratedExpand
Fully charged: Maximizing the potential of cationic polyelectrolytes in applications ranging from membranes to gene delivery through rational design
Polyelectrolytes have found utility across various applications in materials science, from electrochemical devices to biotechnology, due to their facile processing and tunable properties. ThroughExpand
Self‐Assembly of Aminocyclopropenium Salts: En Route to Deltic Ionic Liquid Crystals
Differential scanning calorimetry, polarizing optical microscopy and X‐ray diffraction provided insight into the unique self‐assembly and nanosegregation of these liquid crystals. Expand
Impact of building block structure on ion transport in cyclopropenium-based polymerized ionic liquids
Cation geometry, size, and polarity all contribute to conductivity in PS-TAC PILs, with highest conductivity from the isopropyl cation geometry.
Cyclopropenium Cationic-Based Covalent Organic Polymer-Enhanced Poly(ethylene oxide) Composite Polymer Electrolyte for All-Solid-State Li-S Battery.
  • Yu Wang, H. Ji, +4 authors Xiongwei Qu
  • Medicine
  • ACS applied materials & interfaces
  • 2021
Padding iCP@TFSI into the PEO matrix not only has a positive influence on both the ionic conductivity and the mechanical capacity of solid-state polymer electrolytes but also increases the stability of the lithium metal anode, which essentially improves the overall cycling ability of all-solid-state Li-S batteries. Expand


A Single Methylene Group in Oligoalkylamine-Based Cationic Polymers and Lipids Promotes Enhanced mRNA Delivery.
Findings reveal a novel fundamental structure-activity relationship for the delivery of mRNA that is independent of the class of mRNA carrier and define a promising new path of exploration in the field of mRNA therapeutics. Expand
Functionalization of poly(amidoamine) dendrimers with hydrophobic chains for improved gene delivery in mesenchymal stem cells.
These new vectors show a remarkable capacity for mediating the internalization of pDNA with minimum cytotoxicity, being this effect positively correlated with the -CH(2)- content present in the hydrophobic corona. Expand
Clickable Poly(ionic liquids): A Materials Platform for Transfection.
A post-polymerization click reaction is reported that provides facile access to trisaminocyclopropenium (TAC) ion-functionalized macromolecules of various architectures, which are the first class of polyelectrolytes that bear a formal charge on carbon. Expand
Linear cationic click polymer for gene delivery: synthesis, biocompatibility, and in vitro transfection.
Two polymers showed good biocompatibility, inducing neither apoptosis nor necrosis at the test concentration and low hemolysis ratio and erythrocyte aggregation, and suggested that the novel cationic CPs could be promising carriers for gene delivery. Expand
The transition from linear to highly branched poly(β-amino ester)s: Branching matters for gene delivery
The findings prove that the A2 + B3 + C2 approach is highly generalizable and flexible for the design and synthesis of HPAEs, which cannot be achieved by the conventional polymerization approach; H PAEs are more efficient vectors in gene transfection than the corresponding LPAEs. Expand
Pentalysine-grafted ROMP polymers for DNA complexation and delivery.
Of particular interest was the excellent cell viability seen in experiments with polyplexes formed from the pentalysine-grafted polymers, the highest molecular weight graft copolymer, polymer 1e. Expand
Biodegradable Highly Branched Poly(β-Amino Ester)s for Targeted Cancer Cell Gene Transfection.
Results show that because of the triggered degradability of the vector and enhanced receptor-mediated cellular uptake of polyplexes, the HPAESS-FA and HPAess-Lac exhibit superior gene transfection capability in specific cancer cells with negligible cytotoxicity, pointing to their promise as targeted vectors for efficient cancer gene therapy. Expand
Influence of Polyplex Formation on the Performance of Star-Shaped Polycationic Transfection Agents for Mammalian Cells
Four star-shaped polycationic structures, all based on (2-dimethylamino) ethyl methacrylate (DMAEMA) building blocks, were investigated for their potential to deliver DNA to adherent and non-adherent mammalian cells and slightly outperformed the covalently linked agents. Expand
Influence of polycation molecular weight on poly(2-dimethylaminoethyl methacrylate)-mediated DNA delivery in vitro.
The molecular weight of PDMAEMA was found to have a dramatic influence on transfection efficiency, and luciferase reporter gene expression increased as a function of increasing molecular weight, which suggested that the intracellular fate of the polyplexes is more important to overall transfections efficiency than barriers to entry, such as polyplex size. Expand
Influence of polymer architecture and molecular weight of poly(2-(dimethylamino)ethyl methacrylate) polycations on transfection efficiency and cell viability in gene delivery.
It is proposed that polymers with a branched architecture and an intermediate molecular weight are the most promising candidates for efficient gene delivery, since they combine low cytotoxicity with acceptable transfection results. Expand