Influence of Serum from Rats with Fulminant Hepatic Failure on Hepatocytes in a Bioartificial Liver System

@article{Ito2004InfluenceOS,
  title={Influence of Serum from Rats with Fulminant Hepatic Failure on Hepatocytes in a Bioartificial Liver System},
  author={Y Ito and Susumu Eguchi and Yukio Kamohara and Hiroyuki Inuo and Kosho Yamanouchi and Sadayuki Okudaira and Katsuhiko Yanaga and Junichiro Furui and Takashi Kanematsu},
  journal={The International Journal of Artificial Organs},
  year={2004},
  volume={27},
  pages={303 - 310}
}
Fulminant hepatic failure (FHF) is a life-threatening condition marked by many excessively increased unmetabolized toxins and growth factors. Recently developed bioartificial liver (BAL) systems containing hepatocytes can be used to treat patients with FHF. However, the behavior of these hepatocytes on exposure to FHF serum in vitro remains unclear. In the present study, we used FHF rat models and the sera from these rats (i.e., FHF serum) contained elevated inflammatory cytokines (TNF-α, IL-1… 
The effect of rat acute-liver-failure plasma on HepaRG cells.
TLDR
HepaRG cells are negatively affected by rat plasma, even of healthy origin, but their ammonia eliminating capacity is relatively resistant, underlining their suitability for BAL application.
Protective effects of ACLF sera on metabolic functions and proliferation of hepatocytes co-cultured with bone marrow MSCs in vitro.
TLDR
ACLF serum does not impair the cell morphology, viability, proliferation and overall metabolic capacities of hepatocyte co-cultured with MSCs in vitro.
Alginate-encapsulated HepG2 cells in a fluidized bed bioreactor maintain function in human liver failure plasma.
TLDR
Data show that in LF plasma, alginate-encapsulated HepG2 cells can maintain viability, and metabolic, synthetic, and detoxificatory activities, indicating that the system can be scaled-up to form the biological component of a bioartificial liver.
Optimized performance of the integrated hepatic cell-loaded cryogel-based bioreactor with intermittent perfusion of acute liver failure plasma.
TLDR
A hybrid bioreactor that integrates a hepatic cell-loaded cryogel disc and an activated carbon cloth in one compact unit is designed with potential application as a bioartificial liver support and its functionality and longevity in the presence of intermittent exposure to ALF plasma is analyzed.
Effects of acute‐liver‐failure‐plasma exposure on hepatic functionality of HepaRG‐AMC‐Bioartificial Liver
  • G. Nibourg, R. Hoekstra, +4 authors R. Chamuleau
  • Biology, Medicine
    Liver international : official journal of the International Association for the Study of the Liver
  • 2013
The AMC‐bioartificial liver loaded with the human hepatoma cell line HepaRG as biocomponent (HepaRG‐AMC‐BAL) has recently proven efficacious in rats with acute liver failure (ALF). However, its
Fabrication of macroporous cryogels as potential hepatocyte carriers for bioartificial liver support.
TLDR
Cryogel-based closed continuous bioreactor systems could maintain HepG2 cells at high density for 7 days and showed the ability of immobilized cells to detoxify circulating plasma obtained from patients with acute on chronic liver failure (ACLF).
Evaluation of liver support systems for preclinical testing by animal trials.
TLDR
Various animal models of acute liver failure are reviewed with respect to their suitability for evaluating liver support systems (LSS) according to envisaged modes of therapy to increase the value of the preclinical testing of LSS.
Human liver cell lines for the AMC-bioartificial liver
De HepaRG-levercellijn is een veelbelovend biologisch component voor het maken van de kunstlever AMC-BAL. Geert Nibourg werkte aan een humane levercellijn die beschikbaar is in ruime hoeveelheden en

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