Influence of E3 region on conditionally replicative adenovirus mediated cytotoxicity in hepatocellular carcinoma cells

  title={Influence of E3 region on conditionally replicative adenovirus mediated cytotoxicity in hepatocellular carcinoma cells},
  author={Qin Lu and De-Hong Yu and Chao Fang and Fang Liu and Xun Ye and Yi Zhao and Jie Qin and Xiaoni Guo and Min Liang and Fang Hu and Hong-Zhuan Chen},
  journal={Cancer Biology \& Therapy},
  pages={1125 - 1132}
Virotherapy employing conditionally replicative adenovirus (CRAd) represents a novel targeted strategy for the hepatocellular carcinoma (HCC) treatment. In this study, we explored the potential influence of E3 region, which encodes several TRAIL-inhibiting proteins (E3-6.7K, E3-10.4K/14.5K and E3-14.7K), on CRAd mediated cytotoxicity to HCC cells. Two E1B-55 kDa-deleted CRAds containing E3 region (Ad.ΔE1B) or no E3 region (Ad.ΔE1B.ΔE3) were fabricated. Ad.ΔE1B.ΔE3 exhibited higher cytocidal… 

Homologous recombination-based adenovirus vector system for tumor cell-specific gene delivery

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An update of what has been accomplished in the field of adenoviral gene therapy and its application in hepatocellular carcinoma treatment is provided to provide an opportunity to improve the quality of life and survival of HCC patients.

Adenovirus E3 proteins help tumors to evade innate and adaptive immune responses

Influence of E3 region on conditionally replicative adenovirus mediated cytotoxicity in hepatocellular carcinoma cells is studied.

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Conditionally replicative adenovirus vector carrying TRAIL gene for enhanced oncolysis of human hepatocellular carcinoma.

  • X. YeQ. Lu H. Chen
  • Biology, Medicine
    International journal of molecular medicine
  • 2005
It is demonstrated that the TRAIL in the context of a CRAd vector was able to increase the oncolytic activity in low-AFP-producing HCC cells in vitro and in vivo.

Assessment of a capsid-modified E1B 55-kDa protein-deficient adenovirus vector for tumor treatment

Results suggest that the chimeric oncolytic adenovirus H101-F35 vector might be a useful candidate for gene therapy of cancer.

ADP-overexpressing adenovirus elicits enhanced cytopathic effect by induction of apoptosis

It is reported that ADP-overexpressing Ad markedly enhanced cytolytic effect against all tumor cell lines tested, but did not increase cytopathic effect in normal skin fibroblast, BJ.

Increased therapeutic efficacy of the prostate-specific oncolytic adenovirus Ad[I/PPT-E1A] by reduction of the insulator size and introduction of the full-length E3 region

It is concluded that Ad[i/PPT-E1A, E3] is a prostate-specific oncolytic adenovirus with a high potential for treating localized prostate cancer.

Adenovirus E1A Oncogene Expression in Tumor Cells Enhances Killing by TNF-Related Apoptosis-Inducing Ligand (TRAIL)1

Expression of the adenovirus serotype 5 (Ad5) E1A oncogene sensitizes cells to apoptosis by TNF-α and Fas-ligand. Because TNF-related apoptosis-inducing ligand (TRAIL) kills cells in a similar manner


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Aberrant expression and function of TCF4 in the proliferation of hepatocellular carcinoma cell line BEL-7402

The function of human T-cell transcription factor-4 (TCF4), a key factor of Wnt signaling pathway, on the proliferation of HCC cell line BEL-7402 was studied and indicated that TCF 4 might be another key factor in Wnt pathway involved in HCC cells proliferation and TCF4 could be an effective therapeutic target for suppressing the growth of hepatocellular cancers.

Expression of TNF-related apoptosis-inducing ligand in human hepatocellular carcinoma.

TNF-related apoptosis-inducing ligand, TRAIL, expressed on the surface of HCC cells by cytokines or cytostatic drugs might contribute to an alternative mechanism that enables tumors to evade immune surveillance by inducing apoptosis of activated human lymphocytes.

mad-overexpression down regulates the malignant growth and p53 mediated apoptosis in human hepatocellular carcinoma BEL-7404 cells

Mad proteins may function as a negative regulator antagonizing c-Myc activity in the control of cell growth and apoptosis in human hepatocellular carcinoma BEL-7404 cell line.