Influence of DNA repair by ada and ogt alkyltransferases on the mutational specificity of alkylating agents.

@article{RoldnArjona1994InfluenceOD,
  title={Influence of DNA repair by ada and ogt alkyltransferases on the mutational specificity of alkylating agents.},
  author={Teresa Rold{\'a}n-Arjona and F L Luque-Romero and Rafael R Ariza and Juan Jurado and Carmen Pueyo},
  journal={Molecular carcinogenesis},
  year={1994},
  volume={9 4},
  pages={200-9}
}
We investigated the influence of the alkyltransferases (ATases) encoded by the ada and ogt genes of Escherichia coli on the mutational specificity of alkylating agents. A new mutational assay for selection of supF- mutations in shuttle-vector plasmids was used. Treating plasmid-bearing bacteria with N-methyl-N-nitrosourea (MNU), N-ethyl-N-nitrosourea (ENU), and ethyl methanesulfonate (EMS) dramatically increased the mutation frequency (from 33-fold to 789-fold). The vast majority of mutations… CONTINUE READING