Influence of Bordetella pertussis operon bvgAS on formation and resolution of plasmid-chromosome cointegrates in Escherichia coli K12 mutants with mutational changes on common components of phosphoenolpyruvate-sugar-phosphotransferase system

Abstract

Several plasmids that contain genetically marked variants of the TnBP transposon isolated from Bordetella pertussis have been constructed based on a thermosensitive plasmid. The frequencies of the integration of these plasmids into an E. coli K12 chromosome at a nonpermissive temperature (42°C) have been studied. A decline in the frequency of RSBP-induced and HPr- or enzyme I-deficient cointegrate formation between the plasmid and the K12 bacterial chromosome were detected. The introduction of the bvgAS operon of B. pertussis at the trans-position into the mutant E. coli K12 bacteria restores the frequency of the formation and resolution of RSBP-induced cointegrates.

DOI: 10.3103/S0891416810010027

3 Figures and Tables

Cite this paper

@article{Umyarov2010InfluenceOB, title={Influence of Bordetella pertussis operon bvgAS on formation and resolution of plasmid-chromosome cointegrates in Escherichia coli K12 mutants with mutational changes on common components of phosphoenolpyruvate-sugar-phosphotransferase system}, author={Akhmed Umyarov and L. N. Sinyashina and I. P. Amelina and Kirill A. Datsenko and T. N. Bol’shakova and O. Yu. Dobrynina and Gennady I. Karataev}, journal={Molecular Genetics, Microbiology and Virology}, year={2010}, volume={25}, pages={8-13} }