Influence of 5'-nearest neighbors on the insertion kinetics of the fluorescent nucleotide analog 2-aminopurine by Klenow fragment.

@article{Bloom1993InfluenceO5,
  title={Influence of 5'-nearest neighbors on the insertion kinetics of the fluorescent nucleotide analog 2-aminopurine by Klenow fragment.},
  author={Linda B. Bloom and Mary Rita Otto and Joseph Beechem and Myron F. Goodman},
  journal={Biochemistry},
  year={1993},
  volume={32 41},
  pages={
          11247-58
        }
}
  • Linda B. Bloom, Mary Rita Otto, +1 author Myron F. Goodman
  • Published in Biochemistry 1993
  • Chemistry, Medicine
  • The effects of nearest neighbor interactions between a nucleotide base at the primer 3'-terminus and an incoming deoxyribonucleoside triphosphate on DNA polymerase catalyzed insertion were examined. Kinetics of inserting the fluorescent nucleotide analog 2-aminopurine deoxyribonucleotide (dAPMP) and dAMP opposite a template T by 3'-->5' exonuclease-deficient mutants of Klenow fragment (KF-) were measured on primer/templates of identical sequence except for the base pair at the 3'-primer… CONTINUE READING

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