Inflammatory markers in chronic hepatitis C

Abstract

 To test the hypothesis that inflammation in hepatitis C follows mechanisms common to immune-activated pathways, the distributions of T and B cells, adhesion molecules and transforming growth factor-β (TGF-β) were assessed in liver biopsies with chronic inflammation due to hepatitis C (HCV, n=8) and other causes (non-HCV, n=10). Frozen sections were immunostained using primary antibodies to CD2, CD20, CD4, CD8, intercellular adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM)-1, HLA-DR, lymphocyte function-associated antigen (LFA)-1, and TGF-β. Inflammatory cells positive for each immunophenotypic marker were counted, and positive staining for adhesion molecules, HLA-DR and TGF β was graded in triads and lobules and compared in HCV and non-HCV biopsies. In all biopsies, T cells were more frequent than B cells, both in triads and lobules. CD20+, CD4+, CD8+ and LFA-1+ cells were increased in HCV compared to non-HCV biopsies. Portal lymphoid aggregates were present in 6 of 8 HCV biopsies and 3 of 10 non-HCV biopsies. Aggregates consisted of CD20+, CD4+, CD8+ and LFA-1+ cells, and ICAM-1 and VCAM-1 were increased. Sinusoidal lining cells in HCV biopsies and non-HCV biopsies with inflammation expressed HLA-DR, ICAM-1, and CD4. TGF-β was increased in foci of necrosis. Inflammation in chronic HCV involves common immune-mediated cellular effector pathways and the inflammation in the portal triads represents aggregation of both T and B cells, mediated in part by upregulation of adhesion molecules on portal stromal cells; this is possibly in response to antigens draining from necroinflammatory foci in the lobules. TGF-β is increased in active necroinflammatory foci, but not in portal lymphoid aggregates.

DOI: 10.1007/s004280050086

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@article{Banner1997InflammatoryMI, title={Inflammatory markers in chronic hepatitis C}, author={Barbara F. Banner and Clara Allan and Louis Savas and Stephen B. Baker and Graham F. Barnard and Herbert L. Bonkovsky}, journal={Virchows Archiv}, year={1997}, volume={431}, pages={181-187} }