Inflammatory and oxidative stress markers as indicator of atherogenesis in rats: antioxidants as preventive pharmacological methods.

Abstract

The oxidative process in atherogenesis generated by proinflammatory induction and response to antioxidants vitamins in an experimental model were analyzed. METHODS Male rats were used: (A)Control, (B)Control+vitamin E plus C, (C)Hyperfibrinogenemia and (D)Hyperfibrinogenemia+vitamins E plus C. Hyperfibrinogenemia induced by daily injection of adrenaline (0.1mg/day/rat) for 120 days. TREATMENT 3.42 mg/kg of vitamin E plus 2.14 mg/kg of vitamin C, fifteen days after induction. Vascular histology analyzed by optical microscopy. Fibrinogen, nitrites and superoxide dismutase(SOD) analyzed by spectrophotometry. STATISTICS MANOVA, Hotelling test for post testing, significance level p<0.05. RESULTS (C) group showed higher fibrinogen than (A) and (B)(p<0.001). Compared to (C) group, (D) showed a decrease of fibrinogen(p<0.001). A marked increase in nitrites was found in (C) versus (A), (B) and (D) groups(p<0.001). SOD activity increased in (C) group compared to groups (A) and (B) (p<0.001). In the group (D) an increase of the activity of this enzyme was observed in comparison to groups (C)(p<0.001), (A) and (B) (p<0.0001 in both). The (C) group shown endothelial denudation, thickening of the vascular intima and extracellular matrix enlargement with foam cells(p<0.001). CONCLUSION These results strongly suggest that vitamins E plus C produce regression of inflammatory and oxidative stress processes in this experimental model.

DOI: 10.2174/1871523016666170616121133

Cite this paper

@article{Baez2017InflammatoryAO, title={Inflammatory and oxidative stress markers as indicator of atherogenesis in rats: antioxidants as preventive pharmacological methods.}, author={Maria Del Carmen Baez and Mariana Denise Tar{\'a}n and Mar{\'i}a de La Paz Scribano and Ariel G. Balceda and Carla Buonanotte and Sergio Blencio and Ismael Fonseca and M{\^o}nica Chmeliauskas Moya}, journal={Anti-inflammatory & anti-allergy agents in medicinal chemistry}, year={2017} }