Inflammation-induced miR-802 promotes cell proliferation in cholesteatoma

Abstract

Cholesteatoma is a destructive and abnormal skin growth consisting of keratinizing squamous epithelium in the middle ear. Its molecular mechanisms remain poorly understood. Here, we found that the NF-κB inflammatory signaling pathway was highly activated in cholesteatoma. NF-κB activation increased the expression of microRNA-802 (miR-802) and chromatin immunoprecipitation assays showed that P65 could uniquely bind to miR-802 promoter. miR-802 overexpression promoted keratinocyte cell proliferation and cell cycle progression, while inhibition of miR-802 decreased these effects. From computational analysis and luciferase report assays, miR-802 directly repressed PTEN expression by targeting its 3′-UTR. Our results demonstrate that the NF-κb/miR-802/PTEN signaling pathway plays an important role in the development of cholesteatoma.

DOI: 10.1007/s10529-014-1545-y

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@article{Li2014InflammationinducedMP, title={Inflammation-induced miR-802 promotes cell proliferation in cholesteatoma}, author={Nan Li and Zhao-bing Qin}, journal={Biotechnology Letters}, year={2014}, volume={36}, pages={1753-1759} }